VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
CAR-T Domains
| 名前 | 詳細 | アプリケーションノート | レファレンス | シークエンス |
|---|---|---|---|---|
| CD8-leader | Leader signal peptide of T-cell surface glycoprotein CD8 alpha chain | Directs transport and localization of the protein to the T-cell surface. | Cell. 64:891 (1991); WO2014055771A1; US20160185862A1; US10144770B2 | View |
| CD8-hinge | Hinge region of T-cell surface glycoprotein CD8 alpha chain | Connects scFv and the transmembrane region; enhances tumor recognition efficiency; improves the expansion of CAR-T cells. | Cell. 64:891 (1991); J Immunother. 28:203 (2005); BMJ Open. 6: e013904 (2016); J Hematol Oncol. 10: 68 (2017); Oncoimmunology. 7: e1368604 (2018) | View |
| CD28-hinge | Hinge region of the T-cell surface glycoprotein CD28 | Connects scFv with the transmembrane region providing stability and flexibilty for efficient CAR expression and function; enhances efficiency of tumor recognition; improves expansion of CAR-T cells. | J Immunother. 32:689 (2009); Mol Ther. 25:2452 (2017) | View |
| CD8-TM | Transmembrane region of T-cell surface glycoprotein CD8 alpha chain | Anchors the CAR to the plasma membrane, bridges the extracellular hinge and antigen recognition domains with the intracellular signaling region. | Cell. 64:891 (1991); BMJ Open. 6: e013904 (2016); Oncoimmunology. 7: e1368604 (2018); WO2014055771A1; US20100105136A1 | View |
| CD28-TM | Transmembrane region of T-cell-surface glycoprotein CD28 | Anchors the CAR to the plasma membrane; enhances receptor expression and stability. | Hum Gene Ther. 24:259 (2013); Cancer Res. 74:6383 (2014); WO2014055771A1; US10144770B2 | View |
Stimulatory Domains
| 名前 | 詳細 | アプリケーションノート | レファレンス | シークエンス |
|---|---|---|---|---|
| CD3zeta | Intracellular domain of the T cell receptor-CD3ζ chain | Acts as a stimulatory molecule for activating T cell-mediated immune response. | Cell. 64:891 (1991); Nat Rev Immunol. 2:512 (2002); WO2014055771A1 | View |
Costimulatory Domains
| 名前 | 詳細 | アプリケーションノート | レファレンス | シークエンス |
|---|---|---|---|---|
| CD28 | CD28 intracellular costimulatory domain | Improves overall survival, proliferation and persistence of activated CAR-T cells. | Nat Biotechnol. 20:70 (2002); J Immunother. 32:689 (2009); WO2014055771A1 | View |
| 4-1BB | 4-1BB intracellular costimulatory domain | Improves overall survival, proliferation and persistence of activated CAR-T cells; further enhances cytotoxicity and durability when used in combination with CD28. | Leukemia. 18:676 (2004); Mol Ther. 17:1453 (2009); EP2904106A2 | View |
scFv
| 名前 | 詳細 | アプリケーションノート | レファレンス | シークエンス |
|---|---|---|---|---|
| CD19-scFv | Single-chain variable fragment derived from CD19 antibody | Recognizes human CD19-expressing cells in an antigen-specific manner. | J Immunother. 32:689 (2009); Mol Ther. 17:1453 (2009) | View |